![]() Although the EDC seems to be similar to the intron definition complex (IDC) in composition 5, 6, we do not know if the two complexes differ in their structural organization and how an EDC remodels to span an intron. Support for the exon definition model is largely circumstantial, and biochemical and structural analyses of the exon definition process are limited. However, in order to splice out introns, it was assumed that the exon definition complex (EDC) needs to be remodeled to a cross-intron complex. In the exon definition model, the spliceosome recognizes and assembles across an exon first. ![]() ![]() On the other hand, exon definition 4 prevails in vertebrate, where small exons and large introns are prevalent. In yeast which typically contain small introns and large exons, intron definition, where the spliceosome initially recognizes and assembles across an intron, seems to dominate 3. Thus, how the splicing machinery accurately defines introns and exons remains a fundamental unanswered question. There is, however, a lack of structural and mechanistic understanding of the E complex formation, the earliest event that initiates the splicing cycle. cerevisiae (yeast) spliceosomal complexes 1, 2 provided valuable information on later stages of the splicing cycle. The spliceosome forms sequentially the E, A, Pre-B, B, Bact, B*, C, C*, P, and ILS complexes through the splicing cycle.
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